Vector Transmission of Xylella fastidiosa to Dormant Grape

Glassy-winged Sharpshooter Transmission of Xylella fastidiosa to Plants

Grapevine genotype susceptibility to Xylella fastidiosa does not predict vector transmission success.

For vector-borne diseases, interactions between vector, host, and pathogen can influence patterns of disease spread. In particular, previous studies suggest that host genotype may influence disease dynamics because of differences in susceptibility to the pathogen and, therefore, subsequent vector transmission efficiency from these plants. We tested this hypothesis by using the pathogenic bacte...

Transmission of phage by glassy-winged sharpshooters, a vector of Xylella fastidiosa

Xylella fastidiosa subsp. fastidiosa (Xff) is the causal agent of Pierce's Disease (PD) of grapevines and is vectored by the glassy-winged sharpshooter (GWSS, Homalodisca vitripennis). Previously we have reported the development of a bacteriophage (phage) based biocontrol system for PD, but no information on insect transmission of phages has been reported. Here we communicate that laboratory re...

ANALYSIS OF XYLELLA FASTIDIOSA TRANSPOSON MUTANTS AND DEVELOPMENT OF AN EN PLANTA XYLELLA FASTIDIOSA PLASMID VECTOR Project Leader: Cooperator:

We have developed an autonomously replicating Xylella fastidiosa (Xf)/E. coli plasmid that efficiently transforms Xf; unfortunately this plasmid was not stably maintained in Xf cells en planta or without antibiotic selection in vitro. Another plasmid, containing an Xf native plasmid, a Kan cassette and cloned in pUC18 was also constructed and shown to be unstable without antibiotic selection. 1...

Vector preference for hosts differing in infection status: sharpshooter movement and Xylella fastidiosa transmission

1. Epidemiological theory predicts that vector preference for hosts differing in infection status (i.e. healthy or infected) affects disease dynamics. 2. Numerous studies have documented strong vector preference for or discrimination against infected hosts. However, the significance of these behaviours for pathogen transmission and spread has been poorly described. 3. We conducted a series of c...